Near Field Optical Microscope Applications

Biological applications of near field optical microscopy article pdf available in ieee engineering in medicine and biology magazine 15 1 51 58 february 1996 with 113 reads.

Near field optical microscope applications.

Principle of scanning near field optical microscope and its operation modes. A fundamental principle in diffraction limited optical microscopy requires that the spatial resolution of an image is limited by the wavelength of the incident light and by the numerical apertures of the condenser and objective lens systems. Near field scanning optical microscopy provides a technique for examining specimens with ultra high spatial optical resolution that greatly expands the analytical toolbox of the microscopist. Outline optical near field.

Probes and distance control. A new method for high resolution imaging near field scanning optical microscopy nsom has been developed. Imaging through wavelength refraction and polarization variations snom measurements on algan and uv leds imaging of exciton wave functions time resolved snom measurements. Scanning near field optical microscopy snom based on metal coated adiabatically tapered fibres combined with shear force feedback and operated in illumination mode has proven to be the most.

Near field scanning optical microscopy nsom or scanning near field optical microscopy snom is a microscopy technique for nanostructure investigation that breaks the far field resolution limit by exploiting the properties of evanescent waves in snom the excitation laser light is focused through an aperture with a diameter smaller than the excitation wavelength resulting in an evanescent. Many different methods have been developed in recent years to gain insight into the structure of proteins membranes organelles and cells. Listed in this section are links to resources on the web for near field scanning optical microscopy including university and government laboratories. Near field scanning optical microscopy introduction.

Aperture near field scanning optical microscopy nsom with fluorescence detection gives bio chemical specificity and orientational information in addition to the simultaneously acquired force image.